Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Overexpression of the Endosomal Anion/Proton Exchanger ClC-5 Increases Cell Susceptibility toward Clostridium difficile Toxins TcdA and TcdB

doi: 10.3389/fcimb.2017.00067

Figure Lengend Snippet: ClC-5 and ClC-5 E268Q enhance the internalization of TcdA . HEK293 cells stably transfected with the fluorescent mCherry (blue), ClC-5-mCherry (WT, black) and ClC-5-mCherry E268Q (red) were treated for 45 min on ice followed by a 5-min incubation at 37°C with 200 nM TcdA labeled with an Atto425. TcdA uptake was assessed by the Atto425 fluorescence intensity excited at 425 nm and measured at 440–650 nm. To correct for the different number of cells in the individual experiments, cell density was additionally measured using by absorbance spectrophotometry (Supplementary Figure ). 9 Petri dishes were analyzed for ClC-5 WT and E268Q and 10 for mCherry on two different days. Bars represent means ±SEM at 490 nm emission. * Indicates a significant difference between two data sets. The significance was tested using a two-tailed t -test and significance was set at a P -value of <0.05.

Article Snippet: The bands of fluorescence-tagged ClC-5 and the used protein ladder (Spectra Multicolor Broad Range Protein Ladder, Thermo Scientific) were imaged with a fluorescence scanner (FUSION SL, Vilber Lourat).

Techniques: Stable Transfection, Transfection, Incubation, Labeling, Fluorescence, Spectrophotometry, Two Tailed Test

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Overexpression of the Endosomal Anion/Proton Exchanger ClC-5 Increases Cell Susceptibility toward Clostridium difficile Toxins TcdA and TcdB

doi: 10.3389/fcimb.2017.00067

Figure Lengend Snippet: ClC-5 overexpression promotes endosomal acidification in HEK293 cells. (A) Localization of endocytosed HPTS dye (right panel, green) and ClC-5 mCherry (right panel, red) in live HEK293 cells, with superimposed fluorescence images in red and green resulting in yellow at colocalization. (B) HEK293 cells, stably expressing ClC-5 mCherry, the non-transporting ClC-5 E268Q mCherry, or mCherry alone were incubated with 100 μM HPTS for 24 h and the fluorescence at 510 nm was recorded upon excitation at 350–500 nm in a Horiba Fluorolog spectrophotometer. Intensities are normalized to the isosbestic point at 414 nm. The inset displays the pH obtained by calculating the ratio of the HPTS fluorescence at 510 nm exited at 450 and 390 nm, respectively and converting it to pH using an empirically constructed calibration curve (Supplementary Figure ). Results are presented as mean ±SEM ( n = 4, * p < 0.05). (C) Representative HEK293T cells transiently transfected with ClC-5 mCherry (red) and synapto-pHluorin2 (green). (D) Analysis of vesicular pH of HEK293T cells transiently co-expressing mCherry tagged ClC-5 or ClC-5 E268Q and synapto-pHluorin2 or solely expressing synapto-pHluorin2. The pH was determined using an empirically constructed calibration curve (Supplementary Figure ). Results are shown as means ±SEM. * Indicates a significant difference between two data sets with p < 0.05 ( n = 46 cells for ClC-5, n = 44 for ClC-5 E268Q and n = 34 cells for synapto-pHluorin2). Scale bars correspond to 10 μm.

Article Snippet: The bands of fluorescence-tagged ClC-5 and the used protein ladder (Spectra Multicolor Broad Range Protein Ladder, Thermo Scientific) were imaged with a fluorescence scanner (FUSION SL, Vilber Lourat).

Techniques: Over Expression, Fluorescence, Stable Transfection, Expressing, Incubation, Spectrophotometry, Construct, Transfection

Journal: Sensors (Basel, Switzerland)

Article Title: Large-Scale Indoor Visual–Geometric Multimodal Dataset and Benchmark for Novel View Synthesis

doi: 10.3390/s24175798

Figure Lengend Snippet: Dataset comparison. I/O denotes indoor or outdoor datasets.

Article Snippet: I , Scannet++ , 32.6 m2 , Sony A7IV iPhone13 pro Faro laser scanner , 1920 × 1440 , COLMAP , Mesh Point.

Techniques: Comparison

Journal: Gland Surgery

Article Title: Breast volumetric analysis for aesthetic planning in breast reconstruction: a literature review of techniques

doi: 10.3978/j.issn.2227-684X.2015.10.03

Figure Lengend Snippet: Summary of all 3D surface imaging techniques

Article Snippet: Major limitations of VECTRA systems are its high cost, relatively slow image processing speed, and lack of portability ( ). (ii) Di3D scanner Di3D scanners utilize four high-quality digital SLR (DSLR) cameras, Canon EOS 550D (Canon Inc., Tokyo, Japan), to generate ultra-high quality 3D images ( ).

Techniques: Imaging

Journal: Journal of Digital Imaging

Article Title: Computer-Assisted Nuclear Atypia Scoring of Breast Cancer: a Preliminary Study

doi: 10.1007/s10278-019-00181-8

Figure Lengend Snippet: Confusion matrices

Article Snippet: COMPASS’s performance for images scanned by Aperio scanner Hamamatsu scanner is shown separately.

Techniques: